• MareOfNights@discuss.tchncs.de
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      7 months ago

      I think this basically just tests how heavy chromosomes/DNA-strands are. By that you can see how long they are. The many lines on the side are the reference-ladder.

      8d17261bcfe42286782247ff1229700cba0cfbdd

      The clear lines in the second pic indicate a very clean sample of similar length DNA, while the smears in the first indicate some form of contamination.

      More here

      • notthebees@reddthat.com
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        7 months ago

        Technically that’s gel electrophoresis. It’s basically a way to sort molecules, mainly nucleic molecules like DNA and RNA. It’s a relatively quick and easy way to measure length of chains. The thing on the left is basically a ruler, each stripe corresponds to a different length chain of DNA. The farther away it is from the top, the smaller it is.

        Pcr is polymerase chain reaction, which is how nucleic acids are duplicated in bulk in a lab.

        You mainly use it to compare stuff like genes and ribosomal rna. Genes don’t change too much between strains, and ribosomal RNA is highly conserved (aka barely changes) between species. Basically you have your ruler, some controls +- and then your test sample/s. If your test band lines up with your control band, it’s a match. Or its contaminated.

        The smeary lanes on the left indicate something might be wrong with the gel or the voltage.

      • Ender of Games@sh.itjust.works
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        7 months ago

        More specifically, can you explain the joke? Why am I not worrying (but secretly worrying) about this guy with DNA tests almost the exact same every time?

    • assassinatedbyCIA@lemmy.world
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      7 months ago

      Its some form of blot. Like a western blot or a northern blot or something.

      Edit: the joke is that your blot is kinda bad and doesn’t have clear bands while their blot is really good and strongly identifies the target, dna, rna or protein.